Microscale Thermophoresis (MST) Instrument: NT.LabelFree -Nanotemper

NT.LabelFree - Microscale Thermophoresis (MST) Instrument What is it?
An instrument that uses MST technology is used to quantify molecular interactions between a target and ligand by detecting changes in fluorescence intensity while a temperature gradient is applied over time. The system relies on intrinsic tryptophan fluorescence of the target protein for detection, eliminating the need for fluorescent label.

Manufacturer: Nanotemper

Model Name: Monolith NT.LabelFree

Applications: Interaction study of membrane receptors, soluble proteins, peptides, nucleic acids, sugars, small molecules and macromolecular complexes

Instrument Overview: Monolith uses MST technology to quantify molecular interactions between a target and ligand by detecting changes in fluorescence intensity while a temperature gradient is applied over time. The fluorescent signal comes from the target that has intrinsic fluorescence and becomes an extremely sensitive reporter for the interaction. The binding affinity is automatically determined at the end of each run without additional and lengthy data analysis. The affinity constant (Kd) is calculated from a fitted curve that plots normalized fluorescence against concentration of ligand.

Technical features and specifications:
Samples per run: 16 samples
Fluorescence channels per instrument: 1 (UV)
Fluorescence multiplexing: No
Affinity range: 10nM to mM
Labelling required: No
Tryptophan fluorescence required: Yes
Sample volume: < 4µl
Molecular weight range: 10^1 to 10^7 Daltons
Temperature control: 22°C to 45°C

Charges:
User operated: $200/day, Staff operated: $400/day

Instrument Manufacturer URL:https://www.nanotemper-technologies.com/

Detailed Specifications:
N.A.