16S, ITS sequencing (BIOPOLIS): Bio Rad QX200 Droplet PCR (BIOPOLIS)

equipment

16S, ITS sequencing (BIOPOLIS): Bio Rad QX200 Droplet PCR (BIOPOLIS)

A*STAR Skin Research Lab (A*SRL)

QX200 Droplet Digital PCR What is it?
The QX200 Droplet Digital PCR (ddPCR) System provides absolute quantification of target DNA or RNA molecules for EvaGreen or probe-based digital PCR applications..

Manufacturer: Bio Rad

Model Name: QX200

Applications:
Cancer biomarker studies and copy number variation.
Pathogen detection. Next generation sequencing.
Gene expression analysis.
Environmental monitoring

Charges:NA

Detailed Specifications:
https://https://www.bio-rad.com/en-sg/life-science/digital-pcr/qx200-droplet-digital-pcr-system?ID=MPOQQE4VY&WT_knsh_id=949bef85-664d-43dd-acb7-f96b3933cc98&WT_mc_id=170125000652&WT_srch=1

Submit Enquiry View Page

3D Printing (BIOPOLIS): Bio 3D Printing System (BIOPOLIS)

equipment

3D Printing (BIOPOLIS): Bio 3D Printing System (BIOPOLIS)

A*STAR Skin Research Lab (A*SRL)

What is it?
Bioprinting is the precise depositing of biomaterials, typically living cells in a defined three-dimensional structure. This technology can be used as a tool in high-throughput applications, or to mimic biological systems that are more closer and accurate to the actual living systems for research, testing and diagnosis.

Manufacturer: Regenhu

Model Name: 3D Discovery Evolution

Applications: 3D Bioprinting fabrication of constructs containing living cells

Instrument Overview: The bioprinter contains multiple print-heads and a combination of jetting, dispensing, and electro-writing, which provides unprecedented control of structures at the micro-nano scale. Several accessories including Class II biosafety Cabinet, temperature control unit, light source for photoinitiated gelation, individual temperature control systems have been integrated to support various printing conditions/technologies.

Charges:NA

Detailed Specifications:
https://www.regenhu.com/3dbioprinting-solutions/r-gen-200-3dbioprinter/#versatile-configuration

Submit Enquiry View Page

Brightfield and Widefield: Nikon Ni-E Upright 1

equipment

Brightfield and Widefield: Nikon Ni-E Upright 1

A*STAR Skin Research Lab (A*SRL)

61 Biopolis Drive #06-20 Proteos Singapore 138673

What it is:
The Nikon Ni-E upright microscope is capable of brightfield, DIC and widefield fluorescence microscopy of fixed slide samples.

Manufacturer: Nikon

Model Name: Eclipse Ni-E

Applications: Brightfield & widefield fluorescence light microscopy

Instrument Overview: The Nikon Ni-E upright microscope is equipped with a Nikon DS-Fi3 colour camera for brightfield imaging and a Nikon DS-Qi2 monochrome camera for fluorescence imaging. It has LED light sources and filter sets for fluorescence imaging in DAPI, FITC, Texas Red & Cy5 channels, as well as a DAPI/FITC/TRITC triple bandpass filter set. All functions are controlled by NIS-Elements software.
Technical features and specifications:
Objective lens: 4x, 10x, 20x, 40x oil, 60x oil, 100x oil
Filter cubes: 1. DAPI, 2. FITC/GFP/Alexa488, 3. Texas Red/TRITC/Alexa555/Alexa568, 4. Cy5, 5. DAPI/FITC/ TRITC
Detectors: Colour camera: Nikon DS-Fi3, Monochrome camera: Nikon DS-Qi2
Light source: Transmitted/ brightfield: Nikon LED, Widefield fluorescence: Nikon D-LEDI
Stage: Manual
Software: NIS-Element AR5.41

Charges:
Click HERE for our latest price list.

Instrument Manufacturer URL: https://www.microscope.healthcare.nikon.com/products/upright-microscopes/eclipse-ni-e

Detailed Specifications:
https://tinyurl.com/AMP-Nikon-NiE

Submit Enquiry View Page

Brightfield and Widefield: Nikon Ni-E Upright 2

equipment

Brightfield and Widefield: Nikon Ni-E Upright 2

A*STAR Skin Research Lab (A*SRL)

61 Biopolis Drive #06-20 Proteos Singapore 138673

Submit Enquiry View Page

Confocal Spinning Disk: Spinning Disk 3D FRAP

equipment

Confocal Spinning Disk: Spinning Disk 3D FRAP

A*STAR Skin Research Lab (A*SRL)

61 Biopolis Drive #06-20 Proteos Singapore 138673

What it is:
The spinning disk confocal with 3D-FRAP system is a multi-functional microscope built on a motorized Nikon Eclipse Ti inverted microscope equipped with a stage-top incubator and CO2 control system. It is capable of multi-position 2D and 3D live cell imaging in both spinning disk confocal and widefield modes.

Manufacturer: Various

Model Name:Nikon TiE Body

Applications: 2D and 3D Live-cell imaging, Confocal microscopy

Instrument Overview: The Yokogawa CSU22 confocal scanning unit on this microscope allows for spinning disk confocal imaging in two colours (green and red emission) and DIC, with images captured by a Photometrics Evolve EMCCD camera. An additional iLas 3D-FRAP module allows users to carry out high precision controlled photokinetic experiments (405/491/561 nm) during spinning disk imaging. Widefield fluorescence, DIC and phase contrast imaging can be done on this microscope system using the second detector, a Hamamatsu ORCA Flash4.0 sCMOS camera. For both widefield and spinning disk confocal modes, a piezo z-drive enables fast acquisition of z-stacks for 3D imaging, and the Nikon Perfect Focus System (PFS) ensures long-term timelapse imaging without z drift. Sample holders for 35 mm diameter dishes and chambered coverslips (LabTek II and ibidi) are available.

Technical features and specifications:
Objective lens: 10x, 20x, 40x (oil), 60x (oil)
Filters: DAPI, GFP, TRITC, mCherry, Cy5
Laser Lines: 405m (Photoactivation only), 491nm, 561nm
Cameras: Hamamatsu ORCA Flash4.0 v2 (Widefield), Photometrics Evolve EMCCD (Spinning disk)
Stage: Motorised
Software: Metamorph

Charges:
User operated: $40.20/hour, Staff operated: $96.80/hour

Instrument Manufacturer URL: NA

Detailed Specifications:
https://tinyurl.com/AMP-SpinningDisk3DFrap

Submit Enquiry View Page

Confocal: Leica SP8

equipment

Confocal: Leica SP8

A*STAR Skin Research Lab (A*SRL)

61 Biopolis Drive #06-20 Proteos Singapore 138673

What it is:

Manufacturer: Leica

Model Name: SP8

Applications: Confocal laser scanning microscopy, 3D imaging
Click HERE to Booking System.

Instrument Overview:

Technical features and specifications:
Objective lens:
Filters:
Light sources:
Laser lines:
Confocal imaging: Detectors
Scanners:
Stage:
Software:

Charges:
Click HERE for our latest price list.

Instrument Manufacturer URL:

Detailed Specifications:

Submit Enquiry View Page

Confocal: Olympus FV3000 Inverted

equipment

Confocal: Olympus FV3000 Inverted

A*STAR Skin Research Lab (A*SRL)

61 Biopolis Drive #06-20 Proteos Singapore 138673

What it is:
The FV3000Galvo inverted is a highly motorised confocal laser scanning microscope for multidimensional acquisition (xyzt in multiple channels) of fixed samples.

Manufacturer: Olympus

Model Name: FV3000Galvo Inverted

Applications: Confocal laser scanning microscopy, 3D imaging
Click HERE to Booking System.

Instrument Overview: The extremely fast resonant scanner facilitates rapid acquisition of data which has proven invaluable for acquiring large datasets of thick specimens with large fields of view, requiring the stitching of multiple image stacks. The additional galvo scanner gives the flexibility to carry out more conventional confocal microscopy. The confocal pinhole prevents out-of-focus light from reaching the detector. The FV3000RS is optimized for macro to micro imaging of cells, tissues, and small organisms. The spectral scanner with this system enables high-precision, high-resolution, and high speed spectroscopy (and the acquisition of lambda stacks).

Technical features and specifications:
Objective lens: 1.25x, 10x, 20x, 40x (oil), 60x (oil), 100x
Filters: DAPI, FITC, TRITC, CY5
Light sources: Transmitted (LED), Fluorescence (U-HGLGPS mercury lamp)
Laser Lines: 405nm, 408nm, 561nm, 640nm
Confocal Imaging: 4 PMT Detectors
Scanners: Galvo
Stage: Motorised
Software: FV31S-SW

Charges:
Click HERE for our latest price list.

Instrument Manufacturer URL: NA

Detailed Specifications:
https://tinyurl.com/AMP-FV3000Galv

Submit Enquiry View Page

Confocal: Olympus FV3000RS Inverted

equipment

Confocal: Olympus FV3000RS Inverted

A*STAR Skin Research Lab (A*SRL)

61 Biopolis Drive #06-20 Proteos Singapore 138673

What it is:
The FV3000RS inverted is a highly motorised confocal laser scanning microscope for multidimensional acquisition (xyzt in multiple channels) of fixed samples.

Manufacturer: Olympus

Model Name: FV3000RS

Applications: Confocal laser scanning microscopy, 3D imaging
Click HERE to Booking System.

Instrument Overview: The extremely fast resonant scanner facilitates rapid acquisition of data which has proven invaluable for acquiring large datasets of thick specimens with large fields of view, requiring the stitching of multiple image stacks. The additional galvo scanner gives the flexibility to carry out more conventional confocal microscopy. The confocal pinhole prevents out-of-focus light from reaching the detector. The FV3000RS is optimized for macro to micro imaging of cells, tissues, and small organisms. The spectral scanner with this system enables high-precision, high-resolution, and high speed spectroscopy (and acquisition of lambda stacks).

Technical features and specifications:
Objective lens: 10x, 1.25x, 20x, 40x (oil), 60x (oil), 30x (silicon oil)
Filters: DAPI, FITC, TRITC, CY5
Light sources: Transmitted (LED), Fluorescence (U-HGLGPS mercury lamp)
Laser lines: 405nm, 488nm, 561nm, 650nm
Confocal imaging: 4 PMT Detectors
Scanners: Galvo, Resonant
Stage: Olympus SSU Motorised Stage
Software: FV31S-SW

Charges:
Click HERE for our latest price list.

Instrument Manufacturer URL: https://tinyurl.com/OlyFV3000

Detailed Specifications:
https://tinyurl.com/AMPLM-FV3000RS

Submit Enquiry View Page

Confocal: Olympus FV3000RS Upright

equipment

Confocal: Olympus FV3000RS Upright

A*STAR Skin Research Lab (A*SRL)

61 Biopolis Drive #06-20 Proteos Singapore 138673

What it is:
The FV3000 is a highly motorised confocal laser scanning microscope for multidimensional acquisition (xyzt in multiple channels) of fixed samples.

Manufacturer: Olympus

Model Name: FV3000RS on BX63LF

Applications: Confocal microscopy, high speed zebrafish imaging, Stereomics imaging

Instrument Overview: A highly motorised confocal laser scanning microscope for multidimensional acquisition of fixed samples. The extremely fast resonant scanner facilitates rapid acquisition of data which has proven invaluable for acquiring large datasets of thick specimens with large fields of view, requiring the stitching of multiple image stacks. The additional galvo scanner gives the flexibility to carry out more conventional confocal microscopy. The FV3000RS is optimized for macro to micro imaging of cells and tissues sections. The spectral scanner with this system enables high-precision, high-resolution, and high speed spectroscopy (and the acquisition of lambda stacks). In addition, the high-sensitivity sCMOS camera makes wide field fluorescence imaging fast and flexible while the water dipping lenses can be used for zebra-fish like samples conveniently.
Technical features and specifications:
Objective lens: 1.25x, 10x, 20x, 40x oil, 60x oil, 40x water dipping, 60x water dipping
Filters: 1. DAPI, 2. CFP, 3. FITC/GFP/Alexa488,4. YFP, 5. mCherry, 6. Cy5
Detectors: 2x2CH HSD2(GaAsP), Spectral range: 400nm-800nm (with max.100nm width, 1 nm accuracy)
Light source: Transmitted/ brightfield: LED, Widefield fluorescence: FE300-LED
Stage: H101E1B3 ProScan® stage
Software: For Confocal- FV31S-SWPAD, WideField- cellSens V3.1

Charges:
Click HERE for our latest price list.

Instrument Manufacturer URL: https://www.olympus-lifescience.com/en/laser-scanning/fv3000/

Detailed Specifications:
https://tinyurl.com/AMP-FV3000RS-Upright

Submit Enquiry View Page

Confocal: Zeiss LSM800 Inverted with Env Chamber

equipment

Confocal: Zeiss LSM800 Inverted with Env Chamber

A*STAR Skin Research Lab (A*SRL)

61 Biopolis Drive #06-20 Proteos Singapore 138673

What is it?
A highly motorised confocal laser scanning microscope for multidimensional acquisition (xyzt in multiple channels, tiles, bleaching) of live samples.

Manufacturer: Carl Zeiss

Model Name: LSM800 scan-head sits on Axio Observer.Z1 main body

Applications: Confocal microscopy, 2D and 3D Live-cell imaging, time lapse, bleaching

Instrument Overview: The laser scan confocal is based on an inverted Observer.Z1 microscope, and has all the standard multi-dimensional, multi-position and tiling imaging over time for live samples. In addition, confocal imaging would benefit the most from increased sensitivity, resolution, and speed from 2 sensitive GaAsP detectors , with up to 8 frames/sec with 512 x 512 pixels and up to 6144 x 6144 pixels resolution. The tiling function in the Zen Blue software is quite powerful and allows one to collect multiple images across a large field of view and/or pre-defined positions in multi-well plates using both low and high magnification objectives. In addition, the dedicated experiment-designer module allows complicated imaging process planning.

Technical features and specifications:
Objective lens: 10x, 20x, 40x (water), 63x (water), 63x (oil), 100x (oil)
Filters: DAPI, eGFP, TRITC
Light sources: Transmitted (HAL100), Fluorescence (HXP 120)
Laser lines: 405nm, 488nm, 561nm, 640nm
Environmental Control: PeCon XL Multi S1 DARK LS: heating Unit XL S, CO2 Module S, Temp Module S
Detectors: 2 GaAsP
Software: Zen blue 2.3

Charges:
To be updated

Submit Enquiry View Page

Confocal: Zeiss LSM800 Upright

equipment

Confocal: Zeiss LSM800 Upright

A*STAR Skin Research Lab (A*SRL)

61 Biopolis Drive #06-20 Proteos Singapore 138673

What is it?
A highly motorised confocal laser scanning microscope for multidimensional acquisition (xyzt in multiple channels, tiles, bleaching) of fixed and possibly live samples.

Manufacturer: Carl Zeiss

Model Name: LSM800 scan-head sits on Axio Imager.Z2 main body

Applications: Confocal microscopy, 2D and 3D Live-cell imaging, bleaching

Instrument Overview: The laser scan confocal is based on an upright Axio Imager.Z2 microscope, and has all the standard multi-dimensional, multi-position and tiling imaging over time for fixed samples. Photo Multiplier Tubes (PMTs) detectors make sure the sensitivity is wide from UV to far red. The tiling function in the Zen Blue software is quite powerful and allows one to collect multiple images across a large field of view and/or pre-defined positions in multi-well plates using both low and high magnification objectives. The water-dipping lens makes live-sample imaging possible in the upright configuration.

Technical features and specifications:
Objective lens: 10x, 20x, 40x (water-dipping), 40x (oil), 63x (oil), 100x (oil)
Filters: DAPI, eGFP, TRITC
Light sources: Transmitted (HAL100), Fluorescence (Xcite 120)
Laser lines: 405nm, 488nm, 561nm, 640nm
Detectors: 2 PMT
Software: Zen blue 2.6

Charges:
To be updated

Submit Enquiry View Page

Confocal: Zeiss LSM880 Airyscan Env Chamber (advanced users)

equipment

Confocal: Zeiss LSM880 Airyscan Env Chamber (advanced users)

A*STAR Skin Research Lab (A*SRL)

61 Biopolis Drive #06-20 Proteos Singapore 138673

What is it?
A highly motorised confocal laser scanning microscope for multidimensional acquisition (xyzt in multiple channels, tiles, FCS, FRAP) of live samples with super-resolution detection capability.

Manufacturer: Carl Zeiss

Model Name: LSM880 scan-head sits on Axio Observer.Z1 main body with Airyscan detector

Applications: Confocal microscopy, 2D and 3D Live-cell imaging, time lapse, FCS, FRAP, super-resolution imaging

Instrument Overview: The laser scan confocal is based on an inverted Observer.Z1 microscope, and has all the standard multi-dimensional, multi-position and tiling imaging over time for live samples. In addition, confocal imaging would benefit the most from increased sensitivity, resolution, and speed. Airyscan extends exactly those parameters. Users achieve a 1.7× higher resolution in all spatial dimensions, 140 nm laterally and 400 nm axially. The improved sensitivity leads to better image quality and increased speed. The whole imaging process is possible with standard sample preparation and labeling protocols.

Technical features and specifications:
Objective lens: 10x, 20x, 40x (water), 63x (water), 63x (oil), 100x (oil)
Filters: DAPI, eGFP, TRITC
Light sources: Transmitted (HAL100), Fluorescence (HXP 120)
Laser lines: 405nm, 458nm/488nm/514nm, 555nm, 639nm
Environmental Control: PeCon XL Multi S1 DARK LS: heating Unit XL S, CO2 Module S, Temp Module S
Detectors: 2 PMT, 1 APD, Airyscan
Software: Zen black

Charges:
To be updated

Submit Enquiry View Page

F Techniques: Leica Stellaris

equipment

F Techniques: Leica Stellaris

A*STAR Skin Research Lab (A*SRL)

61 Biopolis Drive #06-20 Proteos Singapore 138673

What it is:
STELLARIS 8 FALCON (FAst Lifetime CONtrast) is the future of functional imaging. Harness the power of fluorescence lifetime to investigate cellular physiology and explore dynamics in living cells. STELLARIS 8 FALCON is a fully integrated solution for Fluorescence Lifetime Imaging (FLIM) and enables video-rate lifetime imaging acquisition for rapid kinetic studies in live cells.

Manufacturer: Leica

Model Name: STELLARIS 8 FALCON

Applications: FILM-FRET, FAST FILM, 3D FILM, Confocal, Live-Cell Imaging
Click HERE to Booking System.

Instrument Overview: In this system, Acousto Optical Beam Splitter (ABOS) technology has replaced the dichroic mirror based emission detection with prism based spectral detection for up to 5 channels simultaneousely. It has a 405nm excitation laser, in addition to tuneable White Light Laser (WLL) for every nm to excite any fluorophore in the range of 440-790 nm, making it suitable for any fluorophore within this range. Its detection range is also suitable for NIR dyes, with detection ranges from 410- 850 nm. The inbuilt in Tau Sense option allows to extract fluorophore lifetimes from confocal image data. The system is also equipped with a stage heater, objective heater and chamber to deliver humidified CO2-rich air.

Technical features and specifications:
Objective lens: 5x, 10x, 20x (Air), 63x (oil), 63x (glycerol), 63x (water)
Filters: 405, CFP, FITC, RHOD
Light sources: Diascopic/Episcopic (LED)
Lasers: 405nm, White light laser with pulse picker (Pulse frequency 80 MHZ) 440-790nm.
Detectors: Power HyD X (Position 2,4), Power HyD S (Position 1), Power HyD R (Position 5)
Spectral detector: 1 Power HyD S detector on the central position 3 (detection range 410-850nm)
Stage: Super Z Galvo Stage
Incubator: Okolab incubating system including humidity sensor & CO2 mixer for chambered slide holder & 35mm glass bottom dish
Software: LASX

Charges:
Click HERE for our latest price list.

Instrument Manufacturer URL: https://www.leica-microsystems.com/products/confocal-microscopes/p/stellaris-8-falcon/

Detailed Specifications:
https://tinyurl.com/AMP-Stellaris

Submit Enquiry View Page

Histology Room 6.12 (BIOPOLIS): L6 Cryostat 3 (Immunos)

equipment

Histology Room 6.12 (BIOPOLIS): L6 Cryostat 3 (Immunos)

A*STAR Skin Research Lab (A*SRL)

What is it?
Leica CM1950 is for fast, reliable and safe cutting accurate frozen sections.

Manufacturer: Leica

Model Name: CM1950

Applications: Processing multiple specimen and performing serial cuts can result in high section numbers. Nevertheless, reproducibility can't be compromised. Permanent attention to detail and many hours in front of the cryostat can be exhausting for the user. The Leica CM1950 cryostat is designed to help with exactly these time-consuming jobs. The low working height and available knee space of the Leica CM1950 cryostat allow the user to work comfortably while sitting. Motorized drive and section counter improve productivity for multiple serial sections. Particularly when working with delicate specimens, e.g. brain samples, the precise specimen orientation and the specimen feed system provide for reproducible sections.

Charges:NA

Detailed Specifications:

Submit Enquiry View Page

Imaging System: Hyperspectral imaging microscope 500 nm – 1100 nm

equipment

Imaging System: Hyperspectral imaging microscope 500 nm – 1100 nm

A*STAR Skin Research Lab (A*SRL)

What is it?

Hyperspectral imaging microscope 500 nm – 1100 nm
Manufacturer: Thorlabs

Model Name: (Asset: 410000022914) Customized

Applications: Microscopy slides examination, fluorescent imaging, histopathology, biological sample examination

Instrument Overview: The instrument is a hyperspectral imaging (HSI) microscope with a detection wavelength range of 500 nm – 1000 nm. It combines the function of an optical spectroscopy and a microscope, i.e. the data contains a spectrum at each image pixel and an image at each wavelength. The obtained data can be analysed with machine learning for classification and visualization of the spatial distribution of a particular material group. It is particularly suitable for multiplexed fluorescence imaging where excitation and emission can be customized. The HSI feature makes sure that cross-talk is minimized.

Technical features and specifications:
Hyperspectral imaging, brightfield microscopy, darkfield microscopy, phase contrast microscopy, 4x, 10x, 20x, 40x, 60x, a wide range of light source from UV to visible.
Contact:
zhang_shuyan@ibb.a-star.edu.sg
Instrument Manufacturer URL: : https://www.thorlabs.com/newgrouppage9.cfm?objectgroup_id=11095

Detailed Specifications:
Hyperspectral imaging, brightfield microscopy, darkfield microscopy, phase contrast microscopy, 4x, 10x, 20x, 40x, 60x, a wide range of light source from UV to visible.

Submit Enquiry View Page

Lightsheet: Ultramicroscope II

equipment

Lightsheet: Ultramicroscope II

A*STAR Skin Research Lab (A*SRL)

61 Biopolis Drive #06-20 Proteos Singapore 138673

What it is:
A light sheet fluorescence microscope that enables you to visualize whole biological systems. It is a second generation selective plane illumination microscope for 3-dimensional imaging.

Manufacturer: Miltenyi BioTec

Model Name: UltraMicroscope II

Applications: Light sheet fluorescence microscopy, Light sheet imaging

Instrument Overview: The UltraMicroscope II allows in vivo imaging as well as the imaging of cleared samples. All optical and mechanical components are compatible to most clearing solutions [DBE, Clarity,etc] and water. It also has a dual-side triple-sheet optics for generating two sets of three laser sheets perpendicular to the direction of detection. Width, Rayleigh length, focus and thickness of the three light sheets can be set by software. Min. thickness: <4.5 μm @ 488 nm for one individual sheet. Focus range: > ±5 mm from center position of the sample for all usable imaging solutions.

Technical features and specifications:
Objective lens: 2x/0.50
Filters: 488nm, 561nm, 640nm, 785nm
Lasers: 488nm, 561nm, 639nm, 785nm
Cameras: Andor Zyla 4.2 Plus sCMOS Camera
Stage: Motorized XYZ-stage for moving imaging chamber and sample holder
Software: LaVision BioTec ImSpector

Charges:
Click HERE for our latest price list.

Instrument Manufacturer URL: https://www.miltenyibiotec.com/SG-en/products/ultramicroscope-ii.html#gref

Submit Enquiry View Page

Live-Cell Imaging: Nikon Ti-E Inverted

equipment

Live-Cell Imaging: Nikon Ti-E Inverted

A*STAR Skin Research Lab (A*SRL)

61 Biopolis Drive #06-20 Proteos Singapore 138673

What it is:
The Nikon widefield live cell imaging system is a multi-functional microscope built on a motorized Nikon Eclipse Ti inverted microscope. It is capable of multi-position imaging of both live and fixed samples in widefield.

Manufacturer: Nikon

Applications: Widefield microscopy, Live cell imaging
Click HERE to Booking System.

Instrument Overview: Widefield fluorescence, DIC and phase contrast imaging can be done on this microscope system using the detector, a Photometrics CoolSnap HQ2 camera. Sample holders for 35 mm diameter dishes, chambered coverslips (LabTek II and ibidi) and multi-well plates are available.

Technical features and specifications:
Objective lens: 4x, 10x, 20x, 40x, 40x (oil), 60x (water), 60x (oil), 100x (oil)
Filters: DAPI, CFP, FITC, YFP, TRITC
Light sources: Transmitted (CoolLED pE-100), Fluorescence (Nikon Intensilight C-HGFIE)
Cameras: Photometrics CoolSnap HQ2 (Widefield)
Stage: Motorised
Software: NIS-Elements AR4.13.05

Charges:
Click HERE for our latest price list.

Instrument Manufacturer URL: NA

Detailed Specifications:
https://tinyurl.com/AMP-NikonTiE

Submit Enquiry View Page

Live-Cell Imaging: Olympus IX-83

equipment

Live-Cell Imaging: Olympus IX-83

A*STAR Skin Research Lab (A*SRL)

61 Biopolis Drive #06-20 Proteos Singapore 138673

What it is:
A dedicated live-cell imaging microscope in multiple positions and multiple channels (both fluorescence and brightfield, including phase and DIC) over long time periods (xyzt in multiple channels).

Manufacturer: Olympus

Model Name: IX83

Applications: Live-cell imaging, Brightfield microscopy, Fluorescence microscopy
Click HERE to Booking System.

Instrument Overview: The system can be used to observe dynamic processes under physiological conditions (temperature, CO2, humidity). Cameras, filters, and stage are all integrated and controlled by CellSense with many features optimized for imaging living cells at high frame rates and/or over long time periods. It is also possible to mark and locate various positions in a culture plate, multi-well plate or on a slide to increase the number of samples acquired.

Technical features and specifications:
Objective lens: 4x,10x,20x,40x, 60x (dry), 60x (oil/silicon oil)
Filters: DAPI, GFP, TRITC, Cy5, CFP, YFP, Cy7
Light sources: Transmitted (monoLED), Fluorescence (CooLED)
Cameras: Hamamatsu ORCA flash 4
Stage: Olympus SSU Motorised
Software: CellSens

Charges:
Click HERE for our latest price list.

Instrument Manufacturer URL: https://tinyurl.com/OlyIX83LC

Detailed Specifications:
https://tinyurl.com/AMPLM-IX83LC

Submit Enquiry View Page

Live-Cell Imaging: Zeiss Axio Observer 7 HCS

equipment

Live-Cell Imaging: Zeiss Axio Observer 7 HCS

A*STAR Skin Research Lab (A*SRL)

61 Biopolis Drive #06-20 Proteos Singapore 138673

What it is:
The Zeiss Axio Observer 7 HCS inverted is designed for fast acquisition of dynamic processes and includes components selected to ensure both stable environmental conditions and highest image quality at different temperatures.

Manufacturer: Zeiss

Model Name: AxioObserver7

Applications: Brightfield & widefield fluorescence light microscopy, live-cell imaging, HCS

Instrument Overview: The Axio Observer 7 is a dedicated live-cell imaging microscope in multiple positions and multiple channels (both fluorescence and birghtfield, including phase and DIC) over long time periods. The microscope is equipped with the latest Definit Focus 3 module, which compensates for the focus drift and keeps samples in focus during long-time-lapse even for most challenging multi-day multi-position time lapse experiments. The fluorescence light source, Colibri 7 with seven excitation wavelengths and filters sets cover the entire visible range, enables fast multispectral imaging. The new design of filter wheel instead of traditional filter cubes makes the switch between different fluorescence channel much faster. The high-magnification long working distance objectives are ideal for samples placed in normal dishes rather than dedicated glass-bottom dishes.
Technical features and specifications:
Objective lens: 5x, 10x, 20x(LD), 40x(LD), 63x oil, 63x water/silicone/glycerine
Filter wheels: 1. DAPI, 2. FITC/GFP/Alexa488, 3. DsRed/TRITC/Alexa555/Alexa568, 4. Cy5/Alexa633/Alexa647, 5. Cy7/Alexa750
Autofocus: Definit Focus 3
Detectors: Colour camera: Axiocam 208 color, Monochrome camera: pco.edge 4.2 - scientific CMOS (sCMOS)
Light source: Transmitted/ brightfield: VIS-LED, Widefield fluorescence: Colibri 7
Stage: Scanning Stage 130x100 STEP
Software: Zen 3.3 pro (blue)

Charges:
Click HERE for our latest price list.

Instrument Manufacturer URL: https://www.micro-shop.zeiss.com/en/us/system/axio+observer-axio+observer+7-inverted+microscopes/10303/

Detailed Specifications:
https://tinyurl.com/Zeiss-AO7-HCS

Submit Enquiry View Page

Mass Spectrometer (BIOPOLIS): DESI-QTOF Waters Synapt XS and HTX-M5 Sprayer

equipment

Mass Spectrometer (BIOPOLIS): DESI-QTOF Waters Synapt XS and HTX-M5 Sprayer

A*STAR Skin Research Lab (A*SRL)

What is it?
Waters DESI XS is for mass spectrometry imaging.

Manufacturer: Waters

Model Name: DESI-QTOF Synapt XS

Applications: DESI™ XS has been designed to deliver improved build quality, reliability and simplicity with innovation in mind. The source is deployable across the Waters HRMS portfolio, bringing to the market a flexible solution and ensuring a consistent, high-level of performance to all imaging customers.

Charges:NA

Detailed Specifications:
https://www.waters.com/waters/en_SG/DESI-XS---Desorption-Electrospray-Ionization-/nav.htm?locale=en_SG&cid=135047466

Submit Enquiry View Page

Multiphoton: LaVision Trim II Multiphoton

equipment

Multiphoton: LaVision Trim II Multiphoton

A*STAR Skin Research Lab (A*SRL)

61 Biopolis Drive #06-20 Proteos Singapore 138673

What it is:
The LaVision Trim II Multiphoton (MP) Microscope combines both inverted and upright microscope with a multiphoton laser and scanhead.

Manufacturer: Olympus

Model Name: LaVision Trim II

Applications: Label-free imaging, 3D imaging, Invtravital imaging, FLIM, FLIM-FRET
Click HERE to Booking System.

Instrument Overview: MP microscopy enables optical sectioning deeper into samples thanks to the long wavelengths of excitation light. MP also allows label free imaging, meaning the auto fluorescence and the second harmonic generation (SHG; e.g. collagen fibers) of the sample can be used for 3D imaging. The inverted orientation is ideal for 3D imaging of cells/organs/tissues. The upright microscope is ideal for intravital imaging. In addition this system has a FLIM X16 bioimaging detector for fluorescence lifetime (FLIM) based applications. The measurement of fluorophore lifetime is based on Time Correlated Single Photon Counting (TCSPC) method. This enables FLIM experiments and FLIM-FRET applications. This system is equipped with both conventional PMT and highly sensitive GaAsP detectors and a Dodt microscopy detector for acquisition of transmitted light images. Both the inverted and upright microscopes are enclosed in cage-incubators to provide temperature, humidity and CO2 control.

Technical features and specifications:
Objective lens: 20x (water), 25x (RI liquids 1.41-1.52), 60x (water)
Filters: Blue, Green, Red
Excition Source: X-Cite 120Q for upright microscope body; Nikon intenslight C-HGFI illuminator for inverted microscope body
Laser Line: 680-1050nm
Detectors: 2 PMT detectors, 2 GaAsp detectors mainly used for auto fluorescence imaging, Becker & Hickl FLIM detector for 2 photon fluorescence lifetime imaging
Stage:
Software: Impspector (LaVision Biotech)

Charges:
Click HERE for our latest price list.

Instrument Manufacturer URL: https://tinyurl.com/TriMScopeII

Detailed Specifications:
https://tinyurl.com/AMP-LVMP

Submit Enquiry View Page

Photoacoustic Imaging: EKSPLA Tunable Nanosecond Laser System NT235

equipment

Photoacoustic Imaging: EKSPLA Tunable Nanosecond Laser System NT235

A*STAR Skin Research Lab (A*SRL)

<What is it?
OPO wavelength tuneable high power pulsed laser

Manufacturer: Ekspla

Model Name: PhotoSonus M-10

Applications: This system can be used to excite biological tissue to generate acoustic signals for photoacoustic imaging

Instrument Overview: High Energy, Mobile, Tunable Wavelength Laser Source for Photoacoustic Imaging

Technical features and specifications:
https://ekspla.com/product/photosonus-m-series-high-energy-tunable-laser-system-for-photoacoustic-imaging/#specifications

Instrument Manufacturer URL: https://ekspla.com/product/photosonus-m-series-high-energy-tunable-laser-system-for-photoacoustic-imaging/#specifications
Contact
Zhang Ruochong zhangrc@ibb.a-star.edu.sg
Detailed Specifications:
https://ekspla.com/product/photosonus-m-series-high-energy-tunable-laser-system-for-photoacoustic-imaging/#specifications

Submit Enquiry View Page

Photoacoustic Tomography System: MSOT inVision 512

equipment

Photoacoustic Tomography System: MSOT inVision 512

A*STAR Skin Research Lab (A*SRL)

What is it?
Photoacoustic imaging device for preclinical applications and clinical application with custom designed handheld probes

Manufacturer: iThera Medical

Model Name: invision 512 Echo

Applications: preclinical applications and clinical applications (skin and breast imaging)

Instrument Overview: The MSOT inVision is a powerful optoacoustic imaging system for small animal imaging. It acquires cross-sectional images, with high resolution, in vivo and in real time. The unique capability of the MSOT inVision to visualize and quantify optoacoustic contrast throughout the whole animal, from head to tail, makes it a very attractive choice for many scientists. Optionally, ultrasound computed tomography can add further insights. With customized 2D or 3D handheld probes, it is used in clinics to image skin and breast cancer specimen.

Technical features and specifications:
The MSOT inVision features 360˚ ring illumination and 270˚ acoustic detection. This wide coverage angle is the basis for the full cross-sectional views of small animals and the unmatched tomographic image quality. The MSOT inVision comes with a custom-design station for animal preparation. Key elements are an injection pad and an animal holder that allows repeatable positioning and anesthesia supply. Following the positioning of the animal, the animal holder is inserted into the imaging chamber of the MSOT inVision. The software then facilitates fully automated image acquisition.Coupled with unique features like a seamless 660-1,300 nm wavelength range and calibrated signal quantification, the inVision users will allow researchers to drive their applications into areas previously not possible.

Instrument Manufacturer URL: https://ithera-medical.com/
Contact
Ghayathri, IBB (ghayathri@ibb.a-star.edu.sg)
Detailed Specifications:
https://ithera-medical.com/products/preclinical-research/msot-invision/

Submit Enquiry View Page

Photoacoustic Tomography System: Raster Scanning Optoacoustic Mesocopy RSOM System

equipment

Photoacoustic Tomography System: Raster Scanning Optoacoustic Mesocopy RSOM System

A*STAR Skin Research Lab (A*SRL)

What is it?
It is a photoacoustic imaging system specifically designed for high resolution skin imaging (lateral resolution ~40 microns) and imaging depth ~1.5-2 mm

Manufacturer: iThera Medical

Model Name: RSOM Explorer ms-C50

Applications: High resolution skin imaging using photoacoustic imaging principle

Instrument Overview: System can be used label free high resolution skim imaging to deduce vascular, structural and functional information in the dermis and epidermis of the skin

Technical features and specifications: details available at https://ithera-medical.com/products/clinical-research/rsom-explorer-c50-research-system/

Instrument Manufacturer URL: https://ithera-medical.com/
Contact
dinish@ibb.a-star.edu.sg
Detailed Specifications: https://ithera-medical.com/products/clinical-research/rsom-explorer-c50-research-system/

Submit Enquiry View Page

Photoacoustic Tomography system: Photoacoustic Tomography PAT System

equipment

Photoacoustic Tomography system: Photoacoustic Tomography PAT System

A*STAR Skin Research Lab (A*SRL)

What is it?
It is a tomographic handheld real-time imaging system specifically designed to acquire soft tissue images with spatial resolution down to 80 µm in vivo and in real time

Manufacturer: iThera Medical

Model Name: MSOT Acuity

Applications: Assessment of suspicious lesions, potentially increasing sensitivity, and specificity of diagnosis for Breast Cancer

Instrument Overview: System can be used as a tumor margin assessment tool to objectively assess the tumor margins in excised lumpectomy specimen.

Technical features and specifications:
Details available at https://ithera-medical.com/products/clinical-research/msot-acuity-echo/
Instrument Manufacturer URL: https://ithera-medical.com/
Contact
Tay Miao Qin, IBB (tay_miao_qin@ibb.a-star.edu.sg)
Detailed Specifications:
https://www.ithera-medical.com/wp-content/uploads/2021/08/iThera-Medical-MSOT-Acuity-Product-Brochure.pdf

Submit Enquiry View Page

Raman Spectrophotometer: Compact Raman system

equipment

Raman Spectrophotometer: Compact Raman system

A*STAR Skin Research Lab (A*SRL)

What is it?
This is in-house built table top Raman system with fiber interphase and 785 nm laser for use in SERS substrate testing.

Manufacturer: Built by TBL, IBB

Model Name: N.A

Applications: This system can be used to acquire Raman spectra of chemical Toxins, food grade chemicals, biochemical and other biofluids (for both pre-clinical and clinical applications)

Instrument Overview: Since it is a in-house built system, this does not have sophisticated software interphase to operate the system for new users and only people who have good understanding of optics basics can operate this system. Hence it cannot be used by non-physicists.

Technical features and specifications:
This system has 78 nm laser with fiber connector between laser to detector. it is operated by multiple windows each one for controlling laser, detector and microscope.
Contact
Jayakumar, IBB (jayakumar_perumal@ibb.a-star.edu.sg)
Detailed Specifications: N.A

Submit Enquiry View Page

Raman Spectrophotometer: Micro Raman Spectrophotometer System

equipment

Raman Spectrophotometer: Micro Raman Spectrophotometer System

A*STAR Skin Research Lab (A*SRL)

What is it?
The inVia microscope comprises a research-grade microscope coupled to a high-performance Raman spectrometer.

Manufacturer: Renishaw

Model Name: inVia

Applications: This system can be used to acquire Raman spectra of chemical Toxins, food grade chemicals, biochemicals, cell lysates, Tissue samples and other materials (for both pre-clinical and clinical applications)

Instrument Overview: The inVia Raman microscope is simple to operate yet delivers outstanding performance and reliable results, for even the most challenging experiments. You can produce both rich, detailed, chemical images and highly specific data from discrete points. With unparalleled flexibility, scientists and engineers, worldwide, trust the inVia microscope.

Technical features and specifications:
The inVia Raman microscope has a comprehensive suite of mapping and imaging techniques. It is mainly used for rapid single scan Spectral acquisitions. It can also be used to generate detailed, information-rich Raman images, of both 2D areas and 3D volumes. This Raman system enables you to achieve a very high level of confocality to understand depth profile of compounds
(this feature is not there in our system) and our system is not “invia Qontor” but just “invia”)
Instrument Manufacturer URL: https://www.renishaw.com
Contact
Jayakumar, IBB (jayakumar_perumal@ibb.a-star.edu.sg)
Detailed Specifications:
https://www.renishaw.com/en/invia-confocal-raman-microscope--6260

Submit Enquiry View Page

Sample Processing Instruments (BIOPOLIS): TECAN Spark Multimode Reader (BIOPOLIS)

equipment

Sample Processing Instruments (BIOPOLIS): TECAN Spark Multimode Reader (BIOPOLIS)

A*STAR Skin Research Lab (A*SRL)

What is it?
TECAN is for scanning multimode microplates.

Manufacturer: TECAN

Model Name: Spark Multimode Reader

Applications: Spark is an all-in-one solution for fast spectral scanning, ELISAs and low volume DNA/protein quantification, featuring fast absorbance measurements with a dedicated High Speed Monochromator.

Charges:NA

Detailed Specifications:
https://lifesciences.tecan.com/multimode-plate-reader

Submit Enquiry View Page

Super Resolution: ONI NanoImager

equipment

Super Resolution: ONI NanoImager

A*STAR Skin Research Lab (A*SRL)

61 Biopolis Drive #06-20 Proteos Singapore 138673

What it is:
A dual-sided lightsheet microscope built in-house that is ideal for imaging 3D biological samples. This microscope combines optical sectioning with high speed and low photo-toxicity.

Manufacturer:

Model Name:

Applications: 3D imaging, Lightsheet microscopy

Instrument Overview: The two lightsheets are generated by scanning of Gaussian beams and provide even illumination on both sides of the sample. Samples can be rotated to be imaged from different viewing angles to achieve isotropic resolution once the images are re-combined. Examples of suitable samples are zebrafish embryo, Drosophila embryo and spheroid/3D organoid culture of 100 um to 500 um in size.

Technical features and specifications:
Objective lens: 10x, 10x (water)
Filters: GFP, RFP, quad-band bandpass
Light sources: Transmitted (LED)
Cameras: Hamamatsu ORCA-Flash4.0 V2 C11440-22CU
Stage: PI 4D (XYZ + rotation)
Software: MicroManager

Charges:
Click HERE for our latest price list.

Instrument Manufacturer URL: NA

Detailed Specifications:
https://tinyurl.com/AMP-ONi-Nanoimager

Submit Enquiry View Page