Imaging Systems: (EARO-HTP) PerkinElmer Opera Phenix (High-Content)

What is it?
A premier laser-light source, fully automated, extremely sensitive confocal, dual camera microscope for demanding high-content applications. The system delivers speed, sensitivity and resolution to capture fine sub-cellular details and lets you find subtle phenotypic changes for simple and complex cellular feature extraction and quantification.

Manufacturer: PerkinElmer

Model Name: Opera Phenix

Applications: High-Content Screening; Phenotypic Screening; FL & BF Microscopy

Instrument Overview: The instrument is compatible with 96- and 384-well sample plates. It has dual cameras for faster image acquisition with minimum crosstalk, four solid-state lasers and three air & three water-immersion objectives for low signal-to-background noise, a dynamic range and high resolution.

Technical features and specifications:

a. Compatibility: Microplates – imaging-qualified clear-bottom 96-/384-well plates
b. Automation: Plate: Handler II Robot for continuous loading up to 28 microplates
c. 6 Objective lens:
1. 10x Air, NA 0.3
2. 20x Air, NA 0.4
3. 40x Air, NA 0.6;
4. 20x Water, NA 1.0
5. 40x Water, NA 1.1
6. 63x Water, 1.15

d. 4 Excitation Lasers [nm]: 1. 405 (50mw),
2. 488 (60mw),
3. 561 (50mw),
4. 640 (40mw)

e. 7 Emission Filters [nm]: 1. Position 1: Cam 1: 435-480, Cam 2: 500-550
2. Position 2: Cam 1: 435-500, Cam 2: 570-630
3. Position 3: Cam 1: 570-630, Cam 2: 650-760
4. Position 4: Cam 1: 650-760

f. Light sources: Transmitted (LED), Fluorescence (Laser)
g. Imaging Modes: Confocal & Widefield
h. Cameras: Two large format sCMOS
i. Stage: Fully automated
j. Software: Harmony (Acquisition & Analysis), Columbus (Storage & Analysis)

Usage Cost:
• Please email us at earo@eddc.sg Instrument Manufacturer URL: https://www.perkinelmer.com/product/opera-phenix-plus-system-hh14001000

Detailed Specifications:
Please send your enquiry for more details.