Level 3 Lab: Octet RED96 System Multichannel Microfluidics

Octet RED96 System Multi Channel Microfluidics Free Protein Interaction System
What is it?
It is a multi-functional, label-free, real-time analysis instrument. It is ideal for measuring concentration of proteins and other biomolecules, measuring kinetics and affinity, and screening protein-protein and protein-small molecule interactions.

Manufacturer: ForteBio

Model Name: Octet RED96 System

Applications: can be used for a wide range of analyses including IgG and other protein titer, bioprocess development, quality analysis, crude antibody screening, epitope binning/mapping, ligand binding assays, small molecule and fragment screening and analysis, elucidating cell signaling mechanisms and infectious disease monitoring.

Instrument Overview: Detects specific proteins and other biomolecules, even from cell culture supernatants and lysates, by direct binding to a wide variety of off-the-shelf biosensors in a simple Dip and Read assay.br>
Technical features and specifications:
Detection Technology: Biolayer Interferometry (BLI) br> Bisensor type: Disposable, single-use fiber optic biosensors with optional reuse by regeneration and/or reracking
Information provided: Kinetic and affinity analysis (kobs, ka , kd, KD); Concentration monitoring (no need for background subtraction); Automated concentration determinations
Data presentation: Plots displaying kinetic binding, equation fits, and residuals of fits; Tabulated kinetic data and data charts; Concentration data analysis; Epitope binning and cross-blocking matrices and trace overlays
Sample types: Proteins, antibodies, peptides, media containing serum, buffers containing DMSO, periplasmic fractions, untreated cell culture supernatants, and crude cell lysates
Sample format: Standard, 96-well, black, flat bottom microplate
Sample volume: 180–220 μL/well; Nondestructive testing
Orbital flow capacity: Static or 100–1500 rpm
Analysis temperature range: (Ambient + 4°C)–40°C, 1°C increments
Throughput: Up to 8 assays in parallel; up to 96 assays per 96-well plate
Analysis time per sample: hIgG quantitation in 2 minutes for 8 samples, ≤ 32 minutes for 96 samples Real-time kinetic binding experiments from 5 minutes to 4 hours
Quantitation range for hIgG: 0.5 µg/mL to 2000 µg/mL at 400 rpm, 0.05 µg/ mL to 100 µg/mL at 1000 rpm
Baseline noise: ≤ 3.5 pm (RMS)
Baseline drift: ≤ 0.1 nm/hour

Charges:
n/a

Instrument Manufacturer URL: https://www.sartorius.com/en

Detailed Specifications:
https://www.sartorius.com/en/products/protein-analysis/octet-label-free-detection-systems/octet-red96e