Level 4 Lab: AKTA Avant 25 Protein Purification System

AKTA Avant 25 Protein Purification System What is it?
ÄKTA avant 25 is a preparative liquid chromatography system intended for media screening, method scouting and process development. Complete solution for fast, high-quality protein separations while maintaining flexibility and reliability. With flow rates up to 25 mL/min, ÄKTA avant 25 system is designed for chromatography resins screening and method optimization.

Manufacturer: GE Healthcare

Model Name: AKTA Avant 25 Protein Purification System

Applications: Protein Purification

Instrument Overview: ÄKTA avant 25 is a preparative chromatography system intended for method and process development, with flow rates up to 25 ml/min. ÄKTA avant 25 system is designed for chromatography resins screening and method optimization. UNICORN™ software is used to control automated processes on ÄKTA avant to increase productivity and efficiency. ÄKTA avant 25 offers a complete solution for fast, high-quality protein separations while maintaining reliability and increasing process knowledge.

Technical features and specifications:
Features of AKTA avant 25: 1.Buffer preparation: this is done automatically to reduce the user’s time and effort. Buffer prepared are consistent in quality and accuracy to minimize variation in the purification parameters. 2.High flow rates: AKTA avant 25 has flow rate of up to 25 ml/min which could shorten the purification time needed. This will increase the throughput to increase cost efficiency in protein purification. 3.Built-in fraction collector: AKTA avant 25 has a built-in fraction collector which enables automatic detection of various labware to ensure the correct labware is used. The fractions are also kept cooled as it is an enclosed chamber. This prevents the protein fraction from degradation. 4.DOE scouting scheme: High throughput buffer screening of bispecific antibodies using design of experiment (DOE) feature to optimize best buffer condition for highest yield and finest purity. 5.Column Logbook: To keep track of every individual column, providing users with column history and performance evaluation data. Various maintenance notifications can be set to sustain performance throughout the lifespan of each column. This would 6.Allow unattended operation: Facilitate long hours run by queuing methods back-to-back. This will minimize labor requirements and increase cost efficiency eventually. 7.Wide range of resins: Extensive resin options for various applications and purification techniques. New and latest resins solution is under continuous development to improve protein purification strategies. 8.Strong local support: Experienced technical and service team to attend and support customers whenever assistance is needed. 9.Proven track record: Huge installation base in market that confers top-notch publications. Protocols could be replicated easily to give consistent protein purity without excessive optimization.
Automatic buffer preparation: Yes
Autosampler injection: No
Depth:710mm
Height: 660mm
Width: 860mm
Pressure range: 0 to 10 Mpa
Conductivity range: 0.01 to 999.99 mS/cm
Flow rate: 0.001 to 25 mL/min (normal range)
Multiple wavelength detection: up to 3 wavelengths
Multistep purification: yes
Pump type: Piston pump, metering type
UV wavelength: 190 to 700nm
pH stability, cleaning-in-place (CIP): 0 to 14
pH stability, operational: 2 to 12
Software: UNICORN™

Charges:
n/a

Instrument Manufacturer URL: https://www.cytivalifesciences.com/en/us

Detailed Specifications: