Microscope: High Content Screening System OPERA PHENIX

PerkinElmer High Content Screening System OPERA PHENIX What is it?
A confocal spinning disk high content imaging microscope. The system can capture images in brightfield, digital phase contrast and multi-channel fluorescent widefield or spinning disc confocal. Can capture slides 96 well/384 well plates with unprecedented speed in 2D and 3D settings.

Manufacturer: Perkin Elmer

Model Name: Opera Phenix

Applications: Fixed-cell assays, Phenotypic screen, 3D cell culture, non-labelled or labelled specimen, small organisms (zebrafish larvae), organoid

Instrument Overview: Dual high sensitivity camera configuration, Spinning disk with increased pinhole-to-pinhole spacing to reduce out-of-focus noise and fluorescence cross talk, PreciScan Intelligent Acquisition module, Water immersion objectives, Laser-based autofocus, software Harmony 4.6. connected to columbus V2.9 image analysis solution with 90TB storage

Technical features and specifications:
Objective lens: 5x(air), 20X (air), 20X (water), 40X (water), 63X (water)zoom
2x 16-bit sCMOS cameras for simultaneous dual colour acquisition (2100 x 2100 pixels)
Solid state Laser 405nm (for DAPI, Hoechst dye), 488nm (for GFP, Alexa 488 dye), 561nm (for RFP, Alexa 555/568, Cy3 dye), 640nm (for Alexa 633/647, cy5 dye)

Charges:
User operated: $0/hour, Staff operated: $60/hour

Instrument Manufacturer URL: Perkin Elmer

Detailed Specifications:
https://www.perkinelmer.com/product/opera-phenix-plus-system-hh14001000