Proteomics: SCIEX 6600 MS

SCIEX Triple TOF 6600 (Proteomics)

What is it?
A high resolution and high sensitivity mass spectrometer that utilizes quadrupole time-of-flight (QTOF) mass analyzer to provide assessment of critical quality attributes (CQA) specifically in the area of biotherapeutic products, new modalities such as gene therapy, cell therapy, and bioprocesses, as well as proteomics analysis and process characterization in alternative protein and isolation/fractionation process for food and nutrition applications.

Manufacturer: SCIEX

Model Name: TripleTOF® 6600 quadrupole time-of-flight (QTOF) mass analyzer

Applications: Biotherapeutics characterizations e.g. Peptide mapping with PTM, N-terminal sequencing, C-terminal sequencing, disulphide linkages, data-Independent SWATH® Acquisition for host cell protein impurities and protein identification and quantitative proteome profiling for food and nutrition application

Instrument Overview: The TripleTOF® 6600 QTOF mass analyser, can be coupled to Waters® nanoACQUITY UPLC® system or NANOLC EKSPERT 425 SYSTEM for LC-MS/MS analysis at nano or micro flow rate to support various data acquisition workflow such as TOF-MS, information dependent acquistion ((IDA) MS/MS, Data independent SWATH® Acquisition with fixed or variable Q1 isolation windows and Targeted MRMHR .

Technical features and specifications:
• Versatile and Fast acquisition speed with no compromise in data resolution
• Complete data-with high-resolution MS/MS data on every detectable analyte
• Advanced workflow capabilities – with high-performance sensitivity
• Enhanced quantitation- with extended linear dynamic range
• Robust spray with the renowned Turbo V™ Source
Specifications:
• Mass range TOF 5 – 40,000 m/z
• Mass selection range Q1 5 – 2250 m/z
• TOF-MS mass resolution and acquisition speed ≥35,000 (FWHM) at 956 m/z, using 10 ms accumulation time
• TOF-MS/MS mass resolution and acquisition speed ≥30,000 (FWHM) at 813 m/z, and ≥25,000 (FWHM) at 195 m/z, using 10 ms accumulation time
• Detector type: 4-channel MCP detector with ADC signal processing for extended dynamic range
• Flexible data acquisition workflows:
o TOF-MS
o Information dependent acquisition (IDA) MS/MS
o Data independent SWATH® Acquisition with fixed or variable Q1 isolation windows
• Targeted MRMHR workflow
Ionization sources IonDrive™ Turbo V, DuoSpray™ Source, each with TurboIonSpray® or atmospheric-pressure chemical ionization (APCI) probes available; NanoSpray® III Source, Digital PicoView NanoSource
Charges:
Project dependent and A*STAR Enterprise guideline

Instrument Manufacturer URL: https://sciex.com/products/mass-spectrometers/qtof-systems/tripletof-systems/tripletof-6600-system

Detailed Specifications:
NA