SPR: Surface Plasmon Resonance Technology BIACORE T200

Surface Plasmon Resonance Technology Unit What is it?
A surface plasmon resonance (SPR) system that provides you with a wide range of high-quality molecular interaction data

Manufacturer: Biacore

Model Name: T200

Applications: Kinetics/affinity characterization, kinetics/affinity screening

Instrument Overview: Biacore T200 is a versatile and precise surface plasmon resonance (SPR) system that provides you with a wide range of high-quality molecular interaction data. Its sensitivity allows the generation of high-quality molecular interaction data — from compounds to viruses. It is useful for comprehensive characterization and comparability studies. Automated run of 384 samples unattended with co-evaluation of up to 5000 samples at one time is possible.

Technical features and specifications:
Affinity range: fM to mM
Analysis temperature: 4°C to 45°C (maximum 20°C below ambient temperature)
Association rate constant (ka): Proteins 103 to 3 × 109 M-1s-1 LMW molecules 103 to 5 × 107 M-1s-1
Buffer selection: Automatic switching between four buffers
Capacity: 1 × 96- or 384-well microplate and up to 33 reagent vials or 78 vials for samples and reagents
Concentration range: > 1 pM
Dissociation rate constant (kd): 10-5 to 1 s-1
Flow cell volume: 60 nL
Flow rate: 1 to 100 μL/min
Injection volume: 2 to 350 μL
Number of flow cells: 4
Refractive index: 1.33 to 1.40
Sample storage temperature: 4°C to 45°C (maximum 15°C below ambient temperature)
Sample type: Small-molecule drug candidates to high-molecular weight proteins (also DNA, RNA, polysaccharides, lipids, cells, and viruses) in various sample environments (e.g., in DMSO-containing buffers, plasma, and serum)
Sample volume min.: Sample volume Injection volume plus 20 to 50 µL (application dependent)
Flow cell height: 40 µm

Charges:
User operated: $400/day, Staff operated: $800/day

Instrument Manufacturer URL:https://www.cytivalifesciences.com/en/us/shop/protein-analysis/spr-label-free-analysis/systems/biacore-t200-p-05644#overview

Detailed Specifications:
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